vector backbone pcag spcas9 gfp u6 grna Search Results


spcas9 d10a expressing vector px335  (Addgene inc)
96
Addgene inc spcas9 d10a expressing vector px335
Spcas9 D10a Expressing Vector Px335, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
spcas9 d10a expressing vector px335 - by Bioz Stars, 2026-10
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px330 cas9 vector  (Addgene inc)
93
Addgene inc px330 cas9 vector
Px330 Cas9 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
px330 cas9 vector - by Bioz Stars, 2026-10
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u6 expression vectors  (Addgene inc)
93
Addgene inc u6 expression vectors
U6 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
u6 expression vectors - by Bioz Stars, 2026-10
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px330 spcas9 hf1 vector  (Addgene inc)
93
Addgene inc px330 spcas9 hf1 vector
Px330 Spcas9 Hf1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
px330 spcas9 hf1 vector - by Bioz Stars, 2026-10
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vector spcas9 px330  (Addgene inc)
96
Addgene inc vector spcas9 px330
Vector Spcas9 Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
vector spcas9 px330 - by Bioz Stars, 2026-10
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crispr cas9 vector  (Addgene inc)
94
Addgene inc crispr cas9 vector
Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
Crispr Cas9 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+backbone+pcag+spcas9+gfp+u6+grna/pCAG-SpCas9-GFP-U6-gRNA+(Plasmid+%2379144)/bio_rxiv__2025__05__26__656092-172-9-7
Average 94 stars, based on 1 article reviews
crispr cas9 vector - by Bioz Stars, 2026-10
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paav u6 grna hsyn mcherry  (Addgene inc)
93
Addgene inc paav u6 grna hsyn mcherry
Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
Paav U6 Grna Hsyn Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
paav u6 grna hsyn mcherry - by Bioz Stars, 2026-10
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pu6 grna scaffold u6 grna scaffold spcas9 2a egfp vector pmak  (Addgene inc)
93
Addgene inc pu6 grna scaffold u6 grna scaffold spcas9 2a egfp vector pmak
Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
Pu6 Grna Scaffold U6 Grna Scaffold Spcas9 2a Egfp Vector Pmak, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pu6 grna scaffold u6 grna scaffold spcas9 2a egfp vector pmak - by Bioz Stars, 2026-10
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spcas9 hairpin u6 sgrna expression plasmid  (Addgene inc)
93
Addgene inc spcas9 hairpin u6 sgrna expression plasmid
Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
Spcas9 Hairpin U6 Sgrna Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
spcas9 hairpin u6 sgrna expression plasmid - by Bioz Stars, 2026-10
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px552 cmv mcherry vector  (Addgene inc)
95
Addgene inc px552 cmv mcherry vector
Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
Px552 Cmv Mcherry Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
px552 cmv mcherry vector - by Bioz Stars, 2026-10
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px330 s pyogenes cas9 vector  (Addgene inc)
93
Addgene inc px330 s pyogenes cas9 vector
Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
Px330 S Pyogenes Cas9 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
px330 s pyogenes cas9 vector - by Bioz Stars, 2026-10
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espcas9 1 1 px330  (Addgene inc)
93
Addgene inc espcas9 1 1 px330
Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using <t>CRISPR/Cas9.</t> B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.
Espcas9 1 1 Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
espcas9 1 1 px330 - by Bioz Stars, 2026-10
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Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using CRISPR/Cas9. B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.

Journal: bioRxiv

Article Title: Syk activation during FcγR-mediated phagocytosis involves both Syk palmitoylation and desulfenylation

doi: 10.1101/2025.05.26.656092

Figure Lengend Snippet: Role of Syk-Cys in phagocytosis. A , the ΔSyk Raw cell line does not express Syk. Western blot of cells following Syk or Firefly luciferase knockout in RAW cells using CRISPR/Cas9. B , Phagocytosis efficiency of Cys to Ser mutants of mSyk following transfection of EGFP-mSyk in ΔSyk RAW cells. Cells were allowed to phagocytose IgG-opsonized 3µm-diameter latex beads for 15 min at 37°C, before counting beads inside and outside cells. See Figure S1 for details.

Article Snippet: To prepare ΔSyk cells we used the Addgene all-in-one CRISPR/Cas9 vector (#79144) as described ( Giuliano et al , 2019 ).

Techniques: Western Blot, Luciferase, Knock-Out, CRISPR, Transfection